YOU SAID:
the reaction mixture was left at room temperature for 4 min, mixed after that, and reacted for a total 30-40 min using a real-time PCR device.
INTO JAPANESE
反応混合物を室温で４分間放置し、その後混合し、リアルタイムＰＣＲ装置を使用して合計３０〜４０分間反応させた。
BACK INTO ENGLISH
The reaction mixture was left at room temperature for 4 minutes, then mixed and allowed to react for a total of 30-40 minutes using a real-time PCR instrument.
INTO JAPANESE
反応混合物を室温で４分間放置し、次いで混合し、リアルタイムＰＣＲ装置を使用して合計３０〜４０分間反応させた。
BACK INTO ENGLISH
The reaction mixture was left at room temperature for 4 minutes, then mixed and allowed to react for a total of 30-40 minutes using a real-time PCR instrument.
You've done this before, haven't you.
