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The equilibrium fluorescence quenching method was used to obtain the binding constant and estimate the active enzyme concentration as described

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平衡蛍光消光法を用いて結合定数を取得し、説明したように、活性酵素濃度を見積もる

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Estimate the enzyme concentration as described, and obtain the coupling constant equilibrium fluorescence quenching method

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前述のように、酵素濃度の推定し、カップリング定数平衡蛍光消光法を取得

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And estimated concentrations of enzymes, as described above, get the coupling constant equilibrium fluorescence quenching method

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酵素の推定濃度前述のように、得る結合定数平衡蛍光消光法と

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And enzyme estimated concentrations above the get binding constant equilibrium fluorescence quenching method

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酵素を得る結合定数平衡蛍光消光法以上の濃度推定

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Estimation of concentration of get enzyme coupling constant equilibrium fluorescence quenching method over

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濃度の推定を得る酵素を結合定数平衡蛍光焼入法

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Fluorescence-quenching method coupling constant equilibrium with enzyme concentration estimate

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蛍光消光法酵素濃度推定値と定数平衡を結合

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Fluorescence quenching method for enzyme concentration estimates combine constant equilibrium

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蛍光消光法酵素濃度の推定値を結合定数平衡の

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Fluorescence quenching method for enzyme concentration estimate of the equilibrium constant

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蛍光消光平衡定数の酵素濃度予測法

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Enzyme concentration prediction method of fluorescence quenching equilibrium constants

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蛍光消光平衡定数の酵素濃度予測法

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Enzyme concentration prediction method of fluorescence quenching equilibrium constants