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he aim of this study is to synthesise superparamagnetic iron oxide nanoparticles conjugated with anti-epidermal growth factor receptor monoclonal antibody (ANTI-EGFR-SPION) and investigate its physicochemical characterisation and biocompatibility as a targeted magnetic resonance imaging (MRI) contrast agent for the EGFR-specific detection in EGFR expressing tumour cells. These particles employed biocompatible polymers, poly(D,L-lactide-co-glycolide) (PLGA) and polyethylene glycol aldehyde (PEG-aldehyde), to increase the half-life of particles in circulation and reduce their side effects. The Fe3O4-loaded PLGA-PEG-aldehyde nanoparticles were prepared by a modified water-in-oil-in-water double emulsion method. The EGFR antibody was conjugated to the surface of SPIONs using the aldehyde-amine reaction. Synthesised conjugates (nanoprobes) were characterised using Fourier transform infrared spectrophotometry, dynamic light scattering, transmission electron microscopy images, and vibrating-sample magnetometery, and the results showed that the conjugation was successful. The mean diameter of nanoprobes was about 25 nm. These nanoprobes exhibited excellent water-solubility, stability, and biocompatibility. Meanwhile, MR susceptibility test proved that synthesised nanoprobes can be managed for negative contrast enhancement. The results of this study suggested the potential use of these nanoprobes for non-invasive molecular MRI in EGFR detection in the future.

INTO JAPANESE

この研究の目的は、抗表皮成長因子受容体モノクローナル抗体(ANTI-EGFR-SPION)と共役する超常磁性鉄ナノ粒子を合成し、EGFR eにおけるEGFR特異的検出用の標的磁気共鳴画像(MRI)造影剤としての物理化学的特徴化と生体適合性を調べる。

BACK INTO ENGLISH

The purpose of this study is to synthesize paramagnetic iron nanoparticles co-associated with anti-epidermal growth factor receptor monoclonal antibodies (ANTI-EGFR-SPION) to investigate physicochemical compatibility and biocompatibility as target magnetic resonance imaging (MRI) contrast agents for EGFR-specific detection in EGFR e.

INTO JAPANESE

本研究の目的は、抗表皮成長因子受容体モノクローナル抗体(ANTI-EGFR-SPION)と共に関連する常磁性鉄ナノ粒子を合成し、EGFR eにおけるEGFR特異的検出用の標的磁気共鳴画像(MRI)造影剤としての物理化学的適合性および生体適合性を調べる。

BACK INTO ENGLISH

The purpose of this study is to synthesize paramagnetic iron nanoparticles associated with anti-epidermal growth factor receptor monoclonal antibodies (ANTI-EGFR-SPION) to investigate physicochemical and biocompatibility as target magnetic resonance imaging (MRI) contrast agents for EGFR-specific detection in EGFR e.

INTO JAPANESE

本研究の目的は、抗表皮成長因子受容体モノクローナル抗体(ANTI-EGFR-SPION)に関連する常磁性鉄ナノ粒子を合成し、EGFReにおけるEGFR特異的検出用の標的磁気共鳴画像法(MRI)造影剤としての物理化学的および生体適合性を調べる。

BACK INTO ENGLISH

The purpose of this study is to synthesize paramagnetic iron nanoparticles associated with anti-epidermal growth factor receptor monoclonal antibodies (ANTI-EGFR-SPION) to investigate physicochemical and biocompatibility as targeted magnetic resonance imaging (MRI) contrast agents for EGFR-specific detection in EGFRe.

INTO JAPANESE

本研究の目的は、抗表皮成長因子受容体モノクローナル抗体(ANTI-EGFR-SPION)に関連する常磁性鉄ナノ粒子を合成し、EGFReにおけるEGFR特異的検出用の標的磁気共鳴画像法(MRI)造影剤としての物理化学的および生体適合性を調べる。

BACK INTO ENGLISH

The purpose of this study is to synthesize paramagnetic iron nanoparticles associated with anti-epidermal growth factor receptor monoclonal antibodies (ANTI-EGFR-SPION) to investigate physicochemical and biocompatibility as targeted magnetic resonance imaging (MRI) contrast agents for EGFR-specific detection in EGFRe.