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DATA SHEET [I-125] RAT OSTEOCALCIN Catalog No: BT-411R FREEZE ON ARRIVAL Quantity: 5uCi/vial Expiration Date: 11-10-99 Concentration: 10uCi/ml Date: 09-10-99 Specific Activity: 152uCi/ug Lot No: 4110999 Packaging: [I-125] Osteocalcin (rat) is packaged in RIA Buffer solution in a multidose vial. Preparation: Rat Osteocalcin (BT-412) is radioiodinated via a modified Chloramine T method. The [I-125] Rat Osteocalcin is then purified by G25 Sephadex gel filtration in RIA buffer. RIA Analysis: Each lot of BTI [I-125] Rat Osteocalcin is analyzed for binding and displacement utilizing our Rat Osteocalcin antibody (BT-413). A copy of a representative curve is provided with each shipment, but standards must be run in every assay. Product Handling: Pipet out amount of tracer to be used dilute with RIA assay buffer. Store both diluted and undiluted tracer at -20°C. **CAUTION: DO NOT PIPET RADIOACTIVE MATERIALS BY MOUTH** Storage & Undiluted [I-125] Rat Osteocalcin should be stored at -20°C and is Stability: stable for six weeks. Diluted tracer should be stored refrigerated at 4°C and is stable for 2-3 days. Reference: 1. Hunter, W.M. and Greenwood, T.C. Nature 194: 495 (1962). FOR RESEARCH USE ONLY RIA BUFFER Make up in = 2 Liters NaCl 0.1225M @ 58.44mw = 14.32g/2 liters NaH2PO4 0.01M @ 137.99mw = 2.76g/2 liters Na4EDTA 2H2O 0.025M @ 416.08mw = 20.80g/2 liters Tween 20 0.1% (w/v) weigh 2g into rinsed beaker, swirl with water until dissolved, and transfer into RIA bottle with several rinses. Bovine Serum Albumin (RIA Grade) 0.1%: weigh 2g into rinsed beaker, add about 150ml H2O and BSA, cover with parafilm, and stir until dissolved (5 minutes). Transfer, with several rinses, into RIA bottle. Adjust pH to 7.4 with concentrated HCl (about 2.9ml added dropwise). Bring final total volume to 2 liters. Special Note: Avoid all possible contamination with Osteocalcin or samples containing Osteocalcin.

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データ シート [I-125] ラット オステオカルシン カタログ No: BT 411R 凍結の入荷量: 5uCi/バイアル期限: 99/11/10 濃度: 10uCi/ml 日: 99/09/10 特定活動: 152uCi/ug 多くない: 4110999 包装: [I-125] オステオカルシン (ラット) はパッケージの RIA 緩衝液で

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Data sheet [I-125] rat Osteocalcin catalogue No: BT 411R freeze amount: 5 uCi / vial due: 11 / 10 / 99: 10uCi/ml date: 99 / 09 / 10 specific activities: 152uCi/ug no more: 4110999 RIA buffer solution package is a packaging: [I-125] Osteocalcin (rat)

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データ シート [I-125] ラット オステオカルシン カタログ No: BT 411R 凍結量: 5 uCi によるバイアル/: 11/10/99: 10uCi/ml 日: 99/09/10 具体的な活動: 152uCi/ug 以上: 4110999 RIA バッファー ソリューション パッケージは、包装: [I-125] オステオカルシン (ラット)

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Data sheet [I-125] rat Osteocalcin catalogue No BT 411R freeze amount: 5 uCi vials /: 11/10/99: 10uCi/ml date: 99 / 09 / 10 specific activities: 152uCi/ug more: 4110999 RIA buffer solution packages is packaging: [I-125] Osteocalcin (rat).

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データ シート [I-125] ラット オステオカルシン カタログ No BT 411R 凍結量: 5 uCi バイアル/: 99/11/10: 10uCi/ml 日: 99/09/10 特定活動: 152uCi/ug より: 4110999 RIA バッファー ソリューション パッケージの包装: [I-125] オステオカルシン (ラット)。

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Data sheet [I-125] rat Osteocalcin catalogue No BT 411R freeze amount: 5 uCi vials / 99 / 11 / 10: 10uCi/ml: 99 / 09 / 10 specific activities: 152uCi/ug: 4110999 RIA buffer solution package packaging: [I-125] Osteocalcin (rat).

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データ シート [I-125] ラット オステオカルシン カタログ No BT 411R 凍結額: 5, uCi バイアル/99/11/10: 10uCi/ml: 99/09/10 具体的な活動: 152uCi/ug: 4110999 RIA バッファー ソリューション パッケージ包装: [I-125] オステオカルシン (ラット)。

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Data sheet [I-125] rat Osteocalcin catalogue No BT 411R freeze amount: 5, uCi vials / 99 / 11 / 10: 10uCi/ml: 99 / 09 / 10 152uCi/ug: 4110999 RIA buffer solution packaging packaging [I-125] Osteocalcin (rat): specific activities.

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データ シート [I-125] ラット オステオカルシン カタログ No BT 411R 凍結量: 5、uCi バイアル/99/11/10: 10uCi/ml: 99/09/10 152uCi/ug: [I-125] オステオカルシン (ラット) を包装包装 4110999 RIA 緩衝液: 具体的な活動。

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Data sheet [I-125] rat Osteocalcin catalogue No BT 411R freeze amount: 5, uCi vials / 99 / 11 / 10: 10uCi/ml: 99 / 09 / 10 152uCi/ug: [I-125] Osteocalcin (rat) packaging packaging RIA 4110999 buffer solution: for specific activities.

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データ シート [I-125] ラット オステオカルシン カタログ No BT 411R 凍結量: 5、uCi バイアル/99/11/10: 10uCi/ml: 99/09/10 152uCi/ug: [I-125] オステオカルシン (ラット) 包装包装 RIA 4110999 緩衝液: 特定の活動のため。

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Data sheet [I-125] rat Osteocalcin catalogue No BT 411R freeze amount: 5, uCi vials / 99 / 11 / 10: 10uCi/ml: 99 / 09 / 10 152uCi/ug: [I-125] for Osteocalcin (rat) packaging packaging RIA 4110999 buffer solution: certain activities.

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データ シート [I-125] ラット オステオカルシン カタログ No BT 411R 凍結量: 5、uCi バイアル/99/11/10: 10uCi/ml: 99/09/10 152uCi/ug: 包装包装 RIA 4110999 緩衝液オステオカルシン (ラット) の [I-125]: 特定の活動。

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Data sheet [I-125] rat Osteocalcin catalogue No BT 411R freeze amount: 5, uCi vials / 99 / 11 / 10: 10uCi/ml: 99 / 09 / 10 [I-125] 152uCi/ug: packaging packaging RIA 4110999 shock absorbing liquid Osteocalcin (rat): certain activities.

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データ シート [I-125] ラット オステオカルシン カタログ No BT 411R 凍結量: 5、uCi バイアル/99/11/10: 10uCi/ml: 99/09/10 [I-125] 152uCi/ug: 包装包装 RIA 4110999 衝撃吸収液オステオカルシン (ラット): 特定の活動。

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Data sheet [I-125] rat Osteocalcin catalogue No BT 411R freeze amount: 5, uCi vials / 99 / 11 / 10: 10uCi/ml: 99 / 09 / 10 [I-125] 152uCi/ug: packaging packaging RIA 4110999 shock absorbing liquid Osteocalcin (rat): certain activities.

Equilibrium found!

Come on, you can do better than that.

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